This volume of Methods in Enzymology is the companion to volume 502 and looks at Protein Engineering for Therapeutics
Part III: Peptides
1. Stapled Peptides for Intracellular Drug Targets
2. Mapping of vascular zip codes by phage display
3. Engineering cyclic peptide toxins
4. Peptide Discovery using Bacterial Display and Flow Cytometry
Part V: Scaffolds
5. Designed Ankyrin Repeat Proteins (DARPins): From research to
therapy
6. Target-binding proteins based on the tenth human fibronectin
type III domain (10Fn3)
7. Anticalins: small engineered binding proteins based on the
lipocalin scaffold
8. T Cell Receptor Engineering
9. Engineering Knottins as Novel Binding Agents
Part VI: Pharmacokinetics
10. Practical Theoretic Guidance for the Design of Tumor Targeting
Agents
11. Re-Engineering Biopharmaceuticals for Targeted Delivery Across
the Blood-Brain Barrier
12. Engineering and Identifying Supercharged Proteins for
Macromolecule Delivery into Mammalian Cells
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