Table of Contents

Preface xiii

Preparation for Protein Isolation 1

Protein Extraction and Solubilization 27

Protein Concentration Determination 57

Concentrating Protein Solutions 83

Gel Electrophoresis Under Denaturing Conditions 107

Gel Electrophoresis Under Nondenaturing Conditions 155

Isoelectric Focusing and Two-Dimensional Gel Electrophoresis 173

Immunoblotting 195

Ion Exchange Chromatography 231

Gel Filtration Chromatography 271

Affinity Chromatography 301

Hanging Drop Crystallization 353

Appendix 1: Molecular weights of commonly used Chemicals 389

Appendix 2: Molecular weights and Isoelectric Points of Selected Protiens 393

Appendix 3: Ammonium Sulfate Precipitation Table 394

Appendix 4: Spectrophotometer Linearity 397

Appendix 5: Suppliers and Addressed 399

Index 405

About the Author

Daniel M. Bollag and Michael D. Rozycki are the authors of Protein Methods, 2nd Edition, published by Wiley.